@article{JGO8583,
author = {Arif Akbar Bhat and Hilal Ahmad Wani and Ajaz Ahmad Waza and Rawoof Ahmad Malik and Akbar Masood and Showkat Jeelani and Showkat Kadla and Sabhiya Majid},
title = {Diminished expression of MGMT & RASSF1A genes in gastric cancer in ethnic population of Kashmir},
journal = {Journal of Gastrointestinal Oncology},
volume = {7},
number = {6},
year = {2016},
keywords = {},
abstract = {Background: Cancer initiation and progression are accompanied by profound changes in DNA. DNA methylation that was the first epigenetic alterations identified in cancer. DNA hypermethylation at promoter sites is closely associated with down regulation of protein and as major participant in the development and progression of series of human tumors. Therefore we hypothesized that promoter hypermethylation of RASSF1A & MGMT gene could influence susceptibility to gastric cancer (GC) as well, and we conducted this study to test the hypothesis in Kashmiri population.
Methods: A hospital based case-control study; including 200 GC cases and 200 matched controls from patients who went surgical resection. Promoter hypermethylation was determined by Methylation Specific Polymerase chain reaction. The expression of MGMT & RASSF1A protein was examined by Western blotting technique.
Results: Frequency of promoter region hypermethylation of MGMT gene were 46.5% in cases and 5.5% in controls (P},
issn = {2219-679X}, url = {https://jgo.amegroups.org/article/view/8583}
}